Bioremediation of Chromium-Contaminated Groundwater Using Chromate Reductase from Pseudomonas putida: An In Silico Approach

Chromium is a toxic heavy metal abundantly present in the environment, specifically groundwater. The groundwater Saudi Arabia was assessed for presence; chromium detected at high concentration Madinah. Many researchers have used various bioprocesses over last few decades to mitigate Cr(VI) toxicity. genus Pseudomonas member putida widely dispersed natural environment. P. chromate-resistant and has chromate reduction rate. Bioremediation procedures can eradicate most potently metal, Cr(VI), water, air, soil. Chromate reductase (ChrR) bacterial enzyme from that be utilized bioremediation remove environment cost-effective environmentally safe approach. To comprehend role of ChrR reducing Cr(III), thorough sequence analysis followed by constructing models wild-type mutants applying several homology modeling techniques. protein structure quality generated evaluated, best model adopted further optimization employing an energy minimization technique. Molecular docking studies investigated intra-molecular interaction between mutant Cr(VI). Our study novel method determining 3D with convert it less hazardous form (III). Additionally, provides stable mutants: Arg83Trp, Gly124Ile, His127Trp, binding affinity which considered engineering produce efficacious enzymes reduce form.